ccr2 antagonist 4 Search Results


ccr2 antagonist 4  (MedChemExpress)
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MedChemExpress ccr2 antagonist 4
a Sankey diagram depicts the chemokine secretion in the host cells and the intestinal mucosa infected with variant and classical PEDV strains. b The protein expression of CCL2 in the ligated terminal jejunum injected with PEDV and in the jejunum from piglets orally inoculated with PEDV was detected via immunofluorescence analysis. CCL2 and PEDV were immunolabeled with anti-CCL2 mAb (green) and anti-PEDV polyclonal antibody (Red), respectively; the cell nuclei were stained with DAPI (blue). Scale bars, 50 μm. c The protein expression of CCL2 and CCL5 in the medium of Marc-145 cells was detected using ELISA kits. d Schematic of experimental setting used to study the migration of IELs influenced by chemokines induced by PEDV infection. e Chemokines CCL2, CCL5, and the CCL2 inhibitor <t>TC1</t> were added into the medium of the basolateral side. Filters from the co-culture system were determined via CLSM, and the intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) in response to different treatments was shown by a three-dimensional (3D) rendering of representative fields. f Quantitative analysis of transepithelial IELs was performed. The number of transepithelial IELs was counted from five random fields of view at a unit area (0.078 mm 2 ) for each of the three individual filters. g The IELs/IPEC-J2 co-culture model was pretreated with an CCL2 inhibitor TC1 for 2 h (DMSO as a negative control) by upper compartment inoculation, followed by a culture with PEDV-infected or whole inactivated PEDV-treated Marc-145 cells. The intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) was detected via CLSM. h Quantitative analysis of transepithelial IELs in each experimental group. All data are the mean ± SD, and comparisons were performed using one-way ANOVA. * P < 0.05, ** P < 0.01. The results are from at least three different experiments.
Ccr2 Antagonist 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ccr2 inhibitor  (MedChemExpress)
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MedChemExpress ccr2 inhibitor
a Sankey diagram depicts the chemokine secretion in the host cells and the intestinal mucosa infected with variant and classical PEDV strains. b The protein expression of CCL2 in the ligated terminal jejunum injected with PEDV and in the jejunum from piglets orally inoculated with PEDV was detected via immunofluorescence analysis. CCL2 and PEDV were immunolabeled with anti-CCL2 mAb (green) and anti-PEDV polyclonal antibody (Red), respectively; the cell nuclei were stained with DAPI (blue). Scale bars, 50 μm. c The protein expression of CCL2 and CCL5 in the medium of Marc-145 cells was detected using ELISA kits. d Schematic of experimental setting used to study the migration of IELs influenced by chemokines induced by PEDV infection. e Chemokines CCL2, CCL5, and the CCL2 inhibitor <t>TC1</t> were added into the medium of the basolateral side. Filters from the co-culture system were determined via CLSM, and the intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) in response to different treatments was shown by a three-dimensional (3D) rendering of representative fields. f Quantitative analysis of transepithelial IELs was performed. The number of transepithelial IELs was counted from five random fields of view at a unit area (0.078 mm 2 ) for each of the three individual filters. g The IELs/IPEC-J2 co-culture model was pretreated with an CCL2 inhibitor TC1 for 2 h (DMSO as a negative control) by upper compartment inoculation, followed by a culture with PEDV-infected or whole inactivated PEDV-treated Marc-145 cells. The intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) was detected via CLSM. h Quantitative analysis of transepithelial IELs in each experimental group. All data are the mean ± SD, and comparisons were performed using one-way ANOVA. * P < 0.05, ** P < 0.01. The results are from at least three different experiments.
Ccr2 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
ccr2 inhibitor - by Bioz Stars, 2026-03
94/100 stars
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a Sankey diagram depicts the chemokine secretion in the host cells and the intestinal mucosa infected with variant and classical PEDV strains. b The protein expression of CCL2 in the ligated terminal jejunum injected with PEDV and in the jejunum from piglets orally inoculated with PEDV was detected via immunofluorescence analysis. CCL2 and PEDV were immunolabeled with anti-CCL2 mAb (green) and anti-PEDV polyclonal antibody (Red), respectively; the cell nuclei were stained with DAPI (blue). Scale bars, 50 μm. c The protein expression of CCL2 and CCL5 in the medium of Marc-145 cells was detected using ELISA kits. d Schematic of experimental setting used to study the migration of IELs influenced by chemokines induced by PEDV infection. e Chemokines CCL2, CCL5, and the CCL2 inhibitor TC1 were added into the medium of the basolateral side. Filters from the co-culture system were determined via CLSM, and the intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) in response to different treatments was shown by a three-dimensional (3D) rendering of representative fields. f Quantitative analysis of transepithelial IELs was performed. The number of transepithelial IELs was counted from five random fields of view at a unit area (0.078 mm 2 ) for each of the three individual filters. g The IELs/IPEC-J2 co-culture model was pretreated with an CCL2 inhibitor TC1 for 2 h (DMSO as a negative control) by upper compartment inoculation, followed by a culture with PEDV-infected or whole inactivated PEDV-treated Marc-145 cells. The intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) was detected via CLSM. h Quantitative analysis of transepithelial IELs in each experimental group. All data are the mean ± SD, and comparisons were performed using one-way ANOVA. * P < 0.05, ** P < 0.01. The results are from at least three different experiments.

Journal: Communications Biology

Article Title: Porcine intraepithelial lymphocytes undergo migration and produce an antiviral response following intestinal virus infection

doi: 10.1038/s42003-022-03205-2

Figure Lengend Snippet: a Sankey diagram depicts the chemokine secretion in the host cells and the intestinal mucosa infected with variant and classical PEDV strains. b The protein expression of CCL2 in the ligated terminal jejunum injected with PEDV and in the jejunum from piglets orally inoculated with PEDV was detected via immunofluorescence analysis. CCL2 and PEDV were immunolabeled with anti-CCL2 mAb (green) and anti-PEDV polyclonal antibody (Red), respectively; the cell nuclei were stained with DAPI (blue). Scale bars, 50 μm. c The protein expression of CCL2 and CCL5 in the medium of Marc-145 cells was detected using ELISA kits. d Schematic of experimental setting used to study the migration of IELs influenced by chemokines induced by PEDV infection. e Chemokines CCL2, CCL5, and the CCL2 inhibitor TC1 were added into the medium of the basolateral side. Filters from the co-culture system were determined via CLSM, and the intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) in response to different treatments was shown by a three-dimensional (3D) rendering of representative fields. f Quantitative analysis of transepithelial IELs was performed. The number of transepithelial IELs was counted from five random fields of view at a unit area (0.078 mm 2 ) for each of the three individual filters. g The IELs/IPEC-J2 co-culture model was pretreated with an CCL2 inhibitor TC1 for 2 h (DMSO as a negative control) by upper compartment inoculation, followed by a culture with PEDV-infected or whole inactivated PEDV-treated Marc-145 cells. The intraepithelial and transepithelial (DAPI, blue) migration of IELs (CFSE, green) was detected via CLSM. h Quantitative analysis of transepithelial IELs in each experimental group. All data are the mean ± SD, and comparisons were performed using one-way ANOVA. * P < 0.05, ** P < 0.01. The results are from at least three different experiments.

Article Snippet: To inhibit the CCR2 receptor activity of IELs, we used CCR2 antagonist 4 (Teijin compound 1, TC1; MedChemExpress, China).

Techniques: Infection, Variant Assay, Expressing, Injection, Immunofluorescence, Immunolabeling, Staining, Enzyme-linked Immunosorbent Assay, Migration, Co-Culture Assay, Negative Control